Modifying secretion and post-translational processing in insect cells.
نویسندگان
چکیده
The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.
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عنوان ژورنال:
- Current opinion in biotechnology
دوره 10 2 شماره
صفحات -
تاریخ انتشار 1999